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v924r  (Addgene inc)


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    Addgene inc v924r
    V924r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v924r/pcDNA3%2E1_Tiam1-pMagFast2(3x)-iRFP+(Plasmid+%2367299)/pm38117650-203-54-71
    Average 91 stars, based on 1 article reviews
    v924r - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

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    Article Title: Distinct interactions stabilize EGFR dimers and higher-order oligomers in cell membranes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific Cat#: 15140148 Catalase from bovine liver Sigma-Aldrich Inc. Cat#: C9322 Glucose oxidase from Aspergillus niger Sigma-Aldrich Inc. Cat#: G2133 Experimental models: Cell lines Hamster: CHO cells ATCC Cat#: CCL-61 Insect: S. frugiperda Sf9 cells Expression Systems Cat#: 94-001F Human: HeLa cells ATCC Cat#: CCL-2 Oligonucleotides gRNA SHC1: 50-CCCGCTCAGCTCTATCCTGG-30 This study N/A gRNA SHC1: 50-CGCCCGCTCAGCTCTATCCT-30 This study N/A gRNA SHC1: 50-TGTAGCCGCCCGCTCAGCTC - 30 This study N/A gRNA GRB2: 50-CTTAGCAAACAGCGGCACGA-30 This study N/A gRNA GRB2: 50-CAGAGCCAAGGCAGAAGAAA-30 This study N/A gRNA GRB2: 50-TGGCAAAATCCCCAGAGCCA-30 This study N/A Recombinant DNA HA-EGFR in pcDNA 3.1 Valley et al.31 N/A HA-EGFR R84K in pcDNA 3.1 This study N/A HA-EGFR A265V in pcDNA 3.1 This study N/A HA-EGFR (T624V/G625V/G628V/A629V/ A637V/G641V) in pcDNA 3.1 (TM3X) This study N/A HA-EGFR (L664A/V665A/L668A/S671A) in pcDNA 3.1 (JM4X) This study N/A HA-EGFR D671-1186 in pcDNA 3.1: HA-EGFR(DICR) This study N/A N-terminally HaloTagged EGFR in pcDNA3.1 This study N/A N-terminally HaloTagged EGFR(DICR) in pcDNA3.1 This study N/A HA-EGFR (I682Q) in pcDNA 3.1 This study N/A HA-EGFR (V924R) in pcDNA 3.1 This study N/A HA-EGFR (D813N) in pcDNA 3.1: HA-EGFR KinThis study N/A EGFR (Y845F/Y992F/Y1045F/Y1068F/ Y1148F/Y1173F) in pcDNA3.1: EGFR Y7F This study N/A EGFR (Y845F/Y974F/Y992F/Y1045F/ Y1068F/Y1101F/Y1114F/Y1148F/Y1173F) in pcDNA3.1: EGFR Y10F This study N/A EGFR (Y974S/Y992S/Y1045S/Y1068S/ Y1101S/Y1148S/Y1173S) in pcDNA3.1: EGFR Y9S This study N/A HA-EGFR (Y974S/Y992S/Y1045S/Y1068S/ Y1101S/Y1148S/Y1173S) in pcDNA3.1: HA-EGFR Y9S This study N/A HA-EGFR (Y845F/Y974S/Y992S/Y1045S/ Y1068S/Y1101S/Y1148S/Y1173S) in pcDNA3.1: HA-EGFR Y10X This study N/A HA-EGFR D999-1186 in pcDNA 3.1: HAEGFR(DC-Tail) This study N/A (Continued on next page) 16 Cell Reports 43, 113603, January 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER N-terminally HaloTagged EGFR(DC-Tail) in pcDNA3.1 This study N/A pHTC HaloTag CMV-neo Vector Promega Cat#: G7711 pFastbac Vector Kit ThermoFisher Scientific Cat#: 10360014 pFastbac sEGFR Ferguson et al.3 N/A pFastbac EGFR TKD [672–998] Park et al.59 N/A pFastbac EGFR JM-TKD [645–998] Red Brewer et al.9 N/A pFastbac EGFR JM-TKD [645–998], V924R This study N/A pFastbac EGFR TKD [672–998], I682Q This study N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al.74 Addgene Cat#: 48138 Software and algorithms DBSCAN Ester et al.75 N/A SMITE (Single Molecule Imaging Toolbox Extraordinaire) K. Lidke lab76 https://github.com/LidkeLab/smite MATLAB version: 9.13.0 (R2022b) The MathWorks Inc., Natick, MA, USA https://www.mathworks.com Origin 2021b (9.8.5.201) OriginLab Corporation, Northampton, MA, USA https://www.originlab.com/ PyMol Molecular Graphics System (Version 2.5.0) Schrödinger, LLC http://www.pymol.org GraphPad Prism 9.5.1 GraphPad Software, La Jolla California USA https://www.graphpad.com/ ExPASy ProtParam Tool Gasteiger et al.77 https://web.expasy.org/protparam/ Broad Institute gRNA designer Broad Institute https://portals.broadinstitute.org/gppx/ crispick/public Adobe Photoshop 24.2.0 Adobe Inc. https://www.adobe.com/products/ photoshop.html .. REAGENT or RESOURCE SOURCE IDENTIFIER N-terminally HaloTagged EGFR(DC-Tail) in pcDNA3.1 This study N/A pHTC HaloTag CMV-neo Vector Promega Cat#: G7711 pFastbac Vector Kit ThermoFisher Scientific Cat#: 10360014 pFastbac sEGFR Ferguson et al.3 N/A pFastbac EGFR TKD [672–998] Park et al.59 N/A pFastbac EGFR JM-TKD [645–998] Red Brewer et al.9 N/A pFastbac EGFR JM-TKD [645–998], V924R This study N/A pFastbac EGFR TKD [672–998], I682Q This study N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al.74 Addgene Cat#: 48138 Software and algorithms DBSCAN Ester et al.75 N/A SMITE (Single Molecule Imaging Toolbox Extraordinaire) K. Lidke lab76 https://github.com/LidkeLab/smite MATLAB version: 9.13.0 (R2022b) The MathWorks Inc., Natick, MA, USA https://www.mathworks.com Origin 2021b (9.8.5.201) OriginLab Corporation, Northampton, MA, USA https://www.originlab.com/ PyMol Molecular Graphics System (Version 2.5.0) Schrödinger, LLC http://www.pymol.org GraphPad Prism 9.5.1 GraphPad Software, La Jolla California USA https://www.graphpad.com/ ExPASy ProtParam Tool Gasteiger et al.77 https://web.expasy.org/protparam/ Broad Institute gRNA designer Broad Institute https://portals.broadinstitute.org/gppx/ crispick/public Adobe Photoshop 24.2.0 Adobe Inc. https://www.adobe.com/products/ photoshop.html

    Imaging:

    Article Title: Distinct interactions stabilize EGFR dimers and higher-order oligomers in cell membranes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific Cat#: 15140148 Catalase from bovine liver Sigma-Aldrich Inc. Cat#: C9322 Glucose oxidase from Aspergillus niger Sigma-Aldrich Inc. Cat#: G2133 Experimental models: Cell lines Hamster: CHO cells ATCC Cat#: CCL-61 Insect: S. frugiperda Sf9 cells Expression Systems Cat#: 94-001F Human: HeLa cells ATCC Cat#: CCL-2 Oligonucleotides gRNA SHC1: 50-CCCGCTCAGCTCTATCCTGG-30 This study N/A gRNA SHC1: 50-CGCCCGCTCAGCTCTATCCT-30 This study N/A gRNA SHC1: 50-TGTAGCCGCCCGCTCAGCTC - 30 This study N/A gRNA GRB2: 50-CTTAGCAAACAGCGGCACGA-30 This study N/A gRNA GRB2: 50-CAGAGCCAAGGCAGAAGAAA-30 This study N/A gRNA GRB2: 50-TGGCAAAATCCCCAGAGCCA-30 This study N/A Recombinant DNA HA-EGFR in pcDNA 3.1 Valley et al.31 N/A HA-EGFR R84K in pcDNA 3.1 This study N/A HA-EGFR A265V in pcDNA 3.1 This study N/A HA-EGFR (T624V/G625V/G628V/A629V/ A637V/G641V) in pcDNA 3.1 (TM3X) This study N/A HA-EGFR (L664A/V665A/L668A/S671A) in pcDNA 3.1 (JM4X) This study N/A HA-EGFR D671-1186 in pcDNA 3.1: HA-EGFR(DICR) This study N/A N-terminally HaloTagged EGFR in pcDNA3.1 This study N/A N-terminally HaloTagged EGFR(DICR) in pcDNA3.1 This study N/A HA-EGFR (I682Q) in pcDNA 3.1 This study N/A HA-EGFR (V924R) in pcDNA 3.1 This study N/A HA-EGFR (D813N) in pcDNA 3.1: HA-EGFR KinThis study N/A EGFR (Y845F/Y992F/Y1045F/Y1068F/ Y1148F/Y1173F) in pcDNA3.1: EGFR Y7F This study N/A EGFR (Y845F/Y974F/Y992F/Y1045F/ Y1068F/Y1101F/Y1114F/Y1148F/Y1173F) in pcDNA3.1: EGFR Y10F This study N/A EGFR (Y974S/Y992S/Y1045S/Y1068S/ Y1101S/Y1148S/Y1173S) in pcDNA3.1: EGFR Y9S This study N/A HA-EGFR (Y974S/Y992S/Y1045S/Y1068S/ Y1101S/Y1148S/Y1173S) in pcDNA3.1: HA-EGFR Y9S This study N/A HA-EGFR (Y845F/Y974S/Y992S/Y1045S/ Y1068S/Y1101S/Y1148S/Y1173S) in pcDNA3.1: HA-EGFR Y10X This study N/A HA-EGFR D999-1186 in pcDNA 3.1: HAEGFR(DC-Tail) This study N/A (Continued on next page) 16 Cell Reports 43, 113603, January 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER N-terminally HaloTagged EGFR(DC-Tail) in pcDNA3.1 This study N/A pHTC HaloTag CMV-neo Vector Promega Cat#: G7711 pFastbac Vector Kit ThermoFisher Scientific Cat#: 10360014 pFastbac sEGFR Ferguson et al.3 N/A pFastbac EGFR TKD [672–998] Park et al.59 N/A pFastbac EGFR JM-TKD [645–998] Red Brewer et al.9 N/A pFastbac EGFR JM-TKD [645–998], V924R This study N/A pFastbac EGFR TKD [672–998], I682Q This study N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al.74 Addgene Cat#: 48138 Software and algorithms DBSCAN Ester et al.75 N/A SMITE (Single Molecule Imaging Toolbox Extraordinaire) K. Lidke lab76 https://github.com/LidkeLab/smite MATLAB version: 9.13.0 (R2022b) The MathWorks Inc., Natick, MA, USA https://www.mathworks.com Origin 2021b (9.8.5.201) OriginLab Corporation, Northampton, MA, USA https://www.originlab.com/ PyMol Molecular Graphics System (Version 2.5.0) Schrödinger, LLC http://www.pymol.org GraphPad Prism 9.5.1 GraphPad Software, La Jolla California USA https://www.graphpad.com/ ExPASy ProtParam Tool Gasteiger et al.77 https://web.expasy.org/protparam/ Broad Institute gRNA designer Broad Institute https://portals.broadinstitute.org/gppx/ crispick/public Adobe Photoshop 24.2.0 Adobe Inc. https://www.adobe.com/products/ photoshop.html .. REAGENT or RESOURCE SOURCE IDENTIFIER N-terminally HaloTagged EGFR(DC-Tail) in pcDNA3.1 This study N/A pHTC HaloTag CMV-neo Vector Promega Cat#: G7711 pFastbac Vector Kit ThermoFisher Scientific Cat#: 10360014 pFastbac sEGFR Ferguson et al.3 N/A pFastbac EGFR TKD [672–998] Park et al.59 N/A pFastbac EGFR JM-TKD [645–998] Red Brewer et al.9 N/A pFastbac EGFR JM-TKD [645–998], V924R This study N/A pFastbac EGFR TKD [672–998], I682Q This study N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al.74 Addgene Cat#: 48138 Software and algorithms DBSCAN Ester et al.75 N/A SMITE (Single Molecule Imaging Toolbox Extraordinaire) K. Lidke lab76 https://github.com/LidkeLab/smite MATLAB version: 9.13.0 (R2022b) The MathWorks Inc., Natick, MA, USA https://www.mathworks.com Origin 2021b (9.8.5.201) OriginLab Corporation, Northampton, MA, USA https://www.originlab.com/ PyMol Molecular Graphics System (Version 2.5.0) Schrödinger, LLC http://www.pymol.org GraphPad Prism 9.5.1 GraphPad Software, La Jolla California USA https://www.graphpad.com/ ExPASy ProtParam Tool Gasteiger et al.77 https://web.expasy.org/protparam/ Broad Institute gRNA designer Broad Institute https://portals.broadinstitute.org/gppx/ crispick/public Adobe Photoshop 24.2.0 Adobe Inc. https://www.adobe.com/products/ photoshop.html



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